Achieve Labs for Microbiology Simulations (1-Term Access)

Introduction

Lab Safety

Background

Procedure

Experiment 1: Safety Features and Hazards

-Part 1: Safety Features

-Part 2: Hazards

-Part 3: Leaving the Lab

Experiment 2: Dressing for the Lab

-Part 1: Dress Code

-Part 2: Personal Protective Equipment (PPE)

Introduction to Lab Simulations

Introduction to the Virtual Laboratory

Touring the Virtual Laboratory

Experiment 1: Measure a Change in Temperature Following a Chemical Reaction Between HCl and NaOH

Experiment 2: Investigate the Relationship between the Volume and Pressure of a Gas

Experiment 3: Observing a Reaction Between HCl and Zinc

Scientific Method

Background

Procedure

Experiment 1: Find the Best Mouse Diet for Weight Gain

Experiment 2: Replicate the Experiment

Basic Microscopy

Background

Procedure

Experiment 1: Visualizing Cells Using a Microscope

-Part 1: Visualizing Amoeba Cells

-Part 2: Visualizing Spirogyra Cells

-Part 3: Visualizing Cardiac Muscle Cells

-Part 4: Visualizing Bacterial Cells

Experiment 2: Estimating Size

-Part 1: Calculating Total Magnification

-Part 2: Calibration 1

-Part 3: Calibration 2

-Part 4: Estimating the Size of an Onion Epidermis Cell

-Part 5: Estimating the Width of a Blood Cell (Neutrophil)

-Part 6: Estimating the Width of a Blood Vessel

Microbiology

Aseptic Technique

Background

Procedure

Experiment 1: Pouring an Agar Plate

-Part 1: Introduction

-Part 2: Removing Agar

-Part 3: Pouring Agar

-Part 4: Summary

Experiment 2: Making a Streak Plate Using the Aseptic Technique

Cultivation of Bacteria

Background

Procedure

Experiment 1: Bacteria in the Environment

Experiment 2: Isolation of Bacteria

-Part 1: Streak Plate Method of Isolation

-Part 2: Spread Plate Method of Isolation

Staining

Background

Procedure

Experiment 1: Gram Staining

Part 1: Heat-Fixation of the S. aureus Bacterial Slide

Part 2: Gram Staining of S. aureus

Part 3: Identifying S. aureus with the Microscope

Part 4: Repeating the Gram Staining Protocol with E. coli

Experiment 2: Acid-Fast Staining

-Part 1: Heat-Fixation of the E. coli and  M. phlei Bacterial Slides

-Part 2: Acid-Fast Staining of E. coli and M. phlei

Experiment 3: Capsule Staining of K. pneumoniae and S. pneumoniae

Enumeration of Bacteria

Background

Procedure

Experiment 1: Spread Plating of Bacterial Serial Dilutions

Experiment 2: Counting Plate CFUs

Experiment 3: Calculating Sample CFU Density

Motility and Amino Acid Hydrolysis

Background

Procedure

Experiment 1: Motility

Experiment 2: Amino Acid Hydrolysis

Blood

Background

Procedure

Experiment 1: Normal Peripheral Blood

Experiment 2: Diseased Peripheral Blood

Unknown Identification

Background

Procedure

Experiment 1: Gram Stain

-Part 1: Heat-Fixation of the Bacterial Slide

-Part 2: Staining

-Part 3: Identifying the Bacteria with the Microscope

Experiment 2: Starch Hydrolysis Test

Experiment 3: Citrate Test

Experiment 4: Anaerobic Jar

Control of Bacterial Growth

Background

Procedure

Experiment 1: UV Radiation

Experiment 2: Chemical Agent Effects on E. coli

Experiment 3: Pressurized Steam, High-Temperature Sterilization Using an Autoclave

Bacterial Transformation

Background

Procedure

Experiment 1: Bacterial Transformation with an Ampicillin-Resistant Plasmid

Experiment 2: Determining Transformation Success Using Selective Media

Extreme Bacteria

Background

Procedure

Experiment 1: Effect of Temperature on Microbial Growth

Experiment 2: Effect of pH on Microbial Growth

Experiment 3: Effect of Osmotic Pressure on Microbial Growth

Experiment 4: Effect of Oxygen on Microbial Growth

Antibiotic Sensitivity

Background

Procedure

Experiment 1: Performing a Disk Diffusion Test with E. coli

Experiment 2: Performing a Disk Diffusion Test with S. aureus

Experiment 3: Performing a Disk Diffusion Test with MRSA

Bacteria

Background

Procedure

Experiment 1: Performing Gram Staining of S. aureus and E. coli

-Part 1: Heat-Fixation of S. aureus

-Part 2: Gram Staining of S. aureus

-Part 3: Visualizing S. aureus with the Microscope

-Part 4: Repeating the Gram Staining Protocol with the E. coli

Experiment 2: Determining the Susceptibility of Gram-Positive and Gram-Negative Bacteria to Antibiotics

-Part 1: Performing a Disk Diffusion Test with Gram-Positive Bacteria

-Part 2: Repeat the Disk Diffusion Test with Gram-Negative Bacteria

Diversity

Protists

Background

Procedure

Topic 1: Investigating Excavata

-Part 1: Visualizing Euglena

-Part 2: Observing Euglena Motion

Topic 2: Investigating Rhizaria

-Part 1: Visualizing Foraminifera Shells

-Part 2: Observing Foram Motion

-Part 3: Investigating Radiolarians

Topic 3: Investigating Chromalveolata

-Part 1: Investigating Diatoms

-Part 2: Investigating Paramecium

-Part 3: Investigating Stentor

-Part 4: Investigating Dinoflagellates

Topic 4: Investigating Archaeplastida

-Part 1: Investigating Spirogyra

-Part 2: Investigating Volvox

-Part 3: Investigating Seaweed

Topic 5: Investigating Amoebas

Topic 6: Investigating Choanoflagellates

Fungi

Background

Procedure

Topic 1: Investigating Phylum Basidiomycota

-Part 1: Visualizing a Whole Agaricus Mushroom Specimen

-Part 2: Visualizing Agaricus Gills

-Part 3: Visualizing Agaricus Hyphae, Basidia, and Spores

Topic 2: Investigating Phylum Ascomycota

-Part 1: Visualizing Whole Ascomycota Mushroom Specimens

-Part 2: Visualizing Aspergillus

-Topic 3: Investigating Phylum Zygomycota

Chemistry

Acids, Bases, and pH Buffers

Background

Procedure

Experiment 1: Measuring pH by Using the pH Indicator Bromothymol Blue

Experiment 2: The Phosphate Buffer System

-Part 1: Measuring pH Changes Following the Addition of HCl

-Part 2: Measuring pH Changes Following the Addition of NaOH

Experiment 3: Measuring the Buffer Capacity of a Phosphate Buffer

-Part 1: Addition of Acid

-Part 2: Addition of Base

Macromolecules

Biological Molecules

Background

Procedure

Experiment 1: Testing for Reducing Sugars Using Benedict’s Solution

Experiment 2: Testing for Starch Using Lugol’s Iodine

Experiment 3: Testing for Lipids Using Sudan III Solution

Experiment 4: Testing for Proteins Using Biuret Solution

Experiment 5: Testing Various Foods for Reducing Sugars, Starch, Lipids, and Proteins

• Part 1: Testing Potato Juice
• Part 2: Testing Onion Juice
• Part 3: Testing Whole Milk
• Part 4: Testing Skim Milk

Enzymes

Background

Procedure

Experiment 1: Determining the Effect of Temperature on Catalase Activity

• Part 1: Determining the Effect of 10 °C on Catalase Activity
• Part 2: Determining the Effect of 21.5 °C on Catalase Activity
• Part 3: Determining the Effect of 40 °C on Catalase Activity
• Part 4: Determining the Effect of 60 °C on Catalase Activity
• Part 5: Determining the Effect of 80 °C on Catalase Activity

Experiment 2: Determining the Effect of Substrate Concentration on Catalase Activity

• Part 1: Testing the Catalase Activity of Test

 -Tube 1

• Part 2: Testing the Catalase Activity of Test

-Tube 2

• Part 3: Testing the Catalase Activity of Test

-Tube 3

• Part 4: Testing the Catalase Activity of Test

-Tube 4

Experiment 3: Determining the Effect of pH on Catalase Activity

• Part 1: Testing Catalase Activity at pH 2
• Part 2: Testing Catalase Activity at pH 6
• Part 3: Testing Catalase Activity at pH 10

Quantitative Analysis of Enzyme Activity

Background

Procedure

Experiment 1: Creating a Calibration Curve for Starch–Iodine Measurements

• Part 1: Preparing a Set of Three Standards of Known Starch Concentration
• Part 2: Measuring Absorbance of the Three Standards

Experiment 2: Determining the Effect of pH on Amylase Enzyme Activity

• Part 1: Preparing the Reaction Solutions and Measuring Their pH
• Part 2: Measuring Absorbance of Test Tube 1 After an Amylase Hydrolysis Reaction
• Part 3: Measuring Absorbance of Test Tube 2 After an Amylase Hydrolysis Reaction
• Part 4: Measuring Absorbance of Test Tube 3 After an Amylase Hydrolysis Reaction
• Part 5: Measuring Absorbance of Test Tube 4 After an Amylase Hydrolysis Reaction
• Part 6: Measuring Absorbance of Test Tube 5 After an Amylase Hydrolysis Reaction

Experiment 3: Determining the Effect of Temperature on Amylase Enzyme Activity

• Part 1: Determining the Effect of 10 °C on Amylase Enzyme Activity
• Part 2: Determining the Effect of 37 °C on Amylase Enzyme Activity
• Part 3: Determining the Effect of 50 °C on Amylase Enzyme Activity
• Part 4: Determining the Effect of 80 °C onAmylase Enzyme Activity

Cells

Diffusion and Osmosis

Background

Procedure

Experiment 1: Qualitative Evidence for Diffusion

Experiment 2: Quantifying the Relationship Between Concentration Gradient and Osmosis

Experiment 3: Visualizing Osmosis in Living Cells

Expanded Diffusion and Osmosis

Background

Procedure

Experiment 1: Qualitative Evidence for Diffusion

Experiment 2: Observing the Dependence of the Rate of Diffusion on the Concentration Gradient

Experiment 3: Confirming Osmosis by Quantifying Weight Changes and Screening for Protein

• Part 1: Quantifying Weight Changes to Confirm Osmosis
• Part 2: Screening for the Presence of Protein with a Biuret Test to Confirm Osmosis

Experiment 4: Quantifying the Relationship Between Concentration Gradient and Osmosis

Metabolism

Cellular Respiration

Background

Procedure

Experiment 1: Fermentation of Different Sugars by Yeast Cells

• Part 1: Measuring Fermentation of Glucose
• Part 2: Measuring Fermentation of Fructose
• Part 3: Measuring Fermentation of Maltose
• Part 4: Measuring Fermentation of Maltotriose

Genetics

DNA

Background

Procedure

Experiment 1: Running a Gel Electrophoresis of DNA VNTR Fragments

Regulation of Gene Expression

Background

Procedure

Topic 1: Investigating Gene Expression

• Part 1: Transcribing Gene 1
• Part 2: Translating Gene 1
• Part 3: Regulating Gene 1
• Part 4: Transcribing Gene 2
• Part 5: Translating Gene 2
• Part 6: Regulating Gene 2
• Part 7: Transcribing Gene 3
• Part 8: Translating Gene 3
• Part 9: Regulating Gene 3

Topic 2: Investigating mRNA Production

Topic 3: Optimizing Protein Production Using Multiple Cells

Biotechnology

PCR

Background

Procedure

Experiment 1: Running a PCR

Experiment 2: Conducting Gel Electrophoresis

Nucleic Acid Assays

Background

Procedure

Experiment 1: Performing an RNA Extraction from Animal Cells

• Part 1: Resuspending the Pellet Containing RNA
• Part 2: Performing Cell Lysis
• Part 3: Homogenizing RNA
• Part 4: Purifying RNA
• Part 5: Summarizing the RNA Extraction Steps

Experiment 2: Cloning

Experiment 3: Next Generation Sequencing

• Part 1: Understanding Next Generation Sequencing
• Part 2: Preparing the Sample
• Part 3: Loading Components Into the MiSeq
• Part 4: Performing Cluster Generation
• Part 5: Sequencing by Synthesis