Achieve Labs for Microbiology Simulations (1-Term Access)

Achieve Lab Simulations for Microbiology
Brief Table of Contents

Introduction
Goal Setting and Reflection Surveys
Welcome to Achieve

Lab Safety

• Experiment 1: Safety Features and Hazards
• Experiment 2: Dressing for the Lab

Introduction to Lab Simulations

• Experiment 1: Measure a Change in Temperature Following a Chemical Reaction Between HCl and NaOH
• Experiment 2: Investigate the Relationship between the Volume and Pressure of a Gas (includes Smart Worksheet)
• Experiment 3: Observing a Reaction Between HCl and Zinc

Scientific Method

• Experiment 1: Find the Best Mouse Diet for Weight Gain (includes Smart Worksheet)
• Experiment 2: Replicate the Experiment (includes Smart Worksheet)

Basic Microscopy

• Experiment 1: Visualizing Cells Using a Microscope
• Experiment 2: Estimating Size

Aseptic Technique

• Experiment 1: Pouring an Agar Plate
• Experiment 2: Making a Streak Plate Using the Aseptic Technique

Cultivation of Bacteria

• Experiment 1: Bacteria in the Environment
• Experiment 2: Isolation of Bacteria

Staining

• Experiment 1: Gram Staining
• Experiment 2: Acid-Fast Staining
• Experiment 3: Capsule Staining of K. pneumoniae and S. pneumoniae

Enumeration of Bacteria

• Experiment 1: Spread Plating of Bacterial Serial Dilutions (includes Smart Worksheet)
• Experiment 2: Counting Plate CFUs (includes Smart Worksheet)
• Experiment 3: Calculating Sample CFU Density (includes Smart Worksheet)

Motility and Amino Acid Hydrolysis

• Experiment 1: Motility
• Experiment 2: Amino Acid Hydrolysis

Blood

• Experiment 1: Normal Peripheral Blood (includes Smart Worksheet)
• Experiment 2: Diseased Peripheral Blood (includes Smart Worksheet)

Unknown Identification

• Experiment 1: Gram Stain
• Experiment 2: Starch Hydrolysis Test
• Experiment 3: Citrate Test
• Experiment 4: Anaerobic Jar

Control of Bacterial Growth

• Experiment 1: UV Radiation
• Experiment 2: Chemical Agent Effects on E. coli
• Experiment 3: Pressurized Steam, High-Temperature Sterilization Using an Autoclave

Bacterial Transformation

• Experiment 1: Bacterial Transformation with an Ampicillin-Resistant Plasmid (includes Smart Worksheet)
• Experiment 2: Determining Transformation Success Using Selective Media (includes Smart Worksheet)

Extreme Bacteria

• Experiment 1: Effect of Temperature on Microbial Growth
• Experiment 2: Effect of pH on Microbial Growth
• Experiment 3: Effect of Osmotic Pressure on Microbial Growth
• Experiment 4: Effect of Oxygen on Microbial Growth

Antibiotic Sensitivity

• Experiment 1: Performing a Disk Diffusion Test with E. coli
• Experiment 2: Performing a Disk Diffusion Test with S. aureus
• Experiment 3: Performing a Disk Diffusion Test with MRSA

Bacteria

• Experiment 1: Performing Gram Staining of S. aureus and E. coli
• Experiment 2: Determining the Susceptibility of Gram-Positive and Gram-Negative Bacteria to Antibiotics

Protists

• Topic 1: Investigating Excavata
• Topic 2: Investigating Rhizaria
• Topic 3: Investigating Chromalveolata
• Topic 4: Investigating Archaeplastida
• Topic 5: Investigating Amoebas
• Topic 6: Investigating Choanoflagellates

Fungi

• Topic 1: Investigating Phylum Basidiomycota
• Topic 2: Investigating Phylum Ascomycota
• Topic 3: Investigating Phylum Zygomycota

Acids, Bases, and pH Buffers

• Experiment 1: Measuring pH by Using the pH Indicator Bromothymol Blue (includes Smart Worksheet)
• Experiment 2: The Phosphate Buffer System (includes Smart Worksheet)
• Experiment 3: Measuring the Buffer Capacity of a Phosphate Buffer (includes Smart Worksheet)

Biological Molecules

• Experiment 1: Testing for Reducing Sugars Using Benedict’s Solution
• Experiment 2: Testing for Starch Using Lugol’s Iodine
• Experiment 3: Testing for Lipids Using Sudan III Solution
• Experiment 4: Testing for Proteins Using Biuret Solution
• Experiment 5: Testing Various Foods for Reducing Sugars, Starch, Lipids, and Proteins

Enzymes

• Experiment 1: Determining the Effect of Temperature on Catalase Activity (includes Smart Worksheet)
• Experiment 2: Determining the Effect of Substrate Concentration on Catalase Activity (includes Smart Worksheet)
• Experiment 3: Determining the Effect of pH on Catalase Activity (includes Smart Worksheet)

Quantitative Analysis of Enzyme Activity

• Experiment 1: Creating a Calibration Curve for Starch–Iodine Measurements (includes Smart Worksheet)
• Experiment 2: Determining the Effect of pH on Amylase Enzyme Activity (includes Smart Worksheet)
• Experiment 3: Determining the Effect of Temperature on Amylase Enzyme Activity (includes Smart Worksheet)

Diffusion and Osmosis

• Experiment 1: Qualitative Evidence for Diffusion
• Experiment 2: Quantifying the Relationship Between Concentration Gradient and Osmosis (includes Smart Worksheet)
• Experiment 3: Visualizing Osmosis in Living Cells

Expanded Diffusion and Osmosis

• Experiment 1: Qualitative Evidence for Diffusion
• Experiment 2: Observing the Dependence of the Rate of Diffusion on the Concentration Gradient (includes Smart Worksheet)
• Experiment 3: Confirming Osmosis by Quantifying Weight Changes and Screening for Protein
• Experiment 4: Quantifying the Relationship Between Concentration Gradient and Osmosis (includes Smart Worksheet)

Cellular Respiration

• Experiment: Fermentation of Different Sugars by Yeast Cells (includes Smart Worksheet)

DNA

• Experiment: Running a Gel Electrophoresis of DNA VNTR Fragments (includes Smart Worksheet)

Regulation of Gene Expression

• Topic 1: Investigating Gene Expression
• Topic 2: Investigating mRNA Production
• Topic 3: Optimizing Protein Production Using Multiple Cells

PCR

• Experiment 1: Running a PCR
• Experiment 2: Conducting Gel Electrophoresis

Nucleic Acid Assays

• Experiment 1: Performing an RNA Extraction from Animal Cells
• Experiment 2: Cloning
• Experiment 3: Next Generation Sequencing